Jan Bashinski DNA Laboratory / CCI Richmond
This 10-1/2 day (86-hour) course conveys in-depth information on human mitochondrial genome biology, evolution, and inheritance as well as practical experience on analytical methods to type genetic variation in this genome. Guidelines on quality assurance, quality control and statistical interpretation of mitochondrial DNA data are reviewed for case work and human identification (missing persons programs). Class is limited to twelve students.
Jan Bashinski DNA Laboratory Staff and guest lecturers
Lecture, discussion, demonstrations, and exercises
Students will gain laboratory experience on (1) DNA extraction from hair, (2) Real Time quantitative PCR for assessment of nuclear and mitochondrial genome copies, (3) PCR amplification of variable regions HV1 and HV2, (4) Microchip (Agilent)-based quantification of amplified products, (5) cycle sequencing and separation of di-deoxy termination products on the ABI Prism 3100 platform, (6) sequence analysis (Sequencher software) and (7) typing of amplified products by Linear Array (Sequence-specific Oligonucleotides). Participants will obtain an understanding on interpretational issues/guidelines regarding both modes of typing (sequencing and Linear Array) with emphasis on the interpretation of patterns of heteroplasmy, and statistical assessments of frequency estimates of mitochondrial haplotypes. Recent developments in SNP-typing technology for variable sites outside of the HV1 and HV2 regions (coding regions) and applications of mitochondrial DNA typing to animal forensic investigations, will also be covered.
SWGDAM-specified courses; casework or casework in-training experience or approval by the CCI Biology/Program Manager
POST-certified; no cost to California public crime laboratory personnel. A $1,200.00 tuition fee is required for all other public agencies, private sector, or out of state applicants.